Regulatory

Part:BBa_K2088012:Design

Designed by: Shi Kejian   Group: iGEM16_NAU-CHINA   (2016-10-08)


MhbRpt is a complex promoter which can be induced by 3-HBA.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 481
    Illegal AgeI site found at 398
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 176


Design Notes

One bp of G was mutated to the A to avoid the formation of the PstⅠ.


Source

The gene can be gotton from the bacteria M5a1, and the sequence comes from GenBank https://www.ncbi.nlm.nih.gov/nuccore/EU165545.1

References